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Lok Raj Joshi

Lok Raj Joshi

Postdoctoral Research Fellow

Ragon Institute of MGH MIT and HARVARD

Biography

I am a postdoctoral research fellow at Ragon Institute of MGH MIT and Harvard.

Download my resumé.

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Interests

• AAV gene therapy
• Viral immunology
• Vaccine development
• Viral pathogenesis
• Diagnostic assay development
• Viral Bioinformatics

Education

• PhD in Biological and Biomedical Sciences (Molecular Virology), 2021

  Cornell University

• Masters in Biological Science, 2017

  South Dakota State University

• Bachelors in Veterinary Science (DVM), 2013

  Tribhuvan University

Experience

 

 

 

 

 

Graduate Research Assistant

Cornell University

Jun 2019 – Aug 2021 NY

Responsibilites :

• Orf virus based vectored vaccine development
• Monoclonal antibody development
• SARS-CoV-2 pathogenesis
• Poxvirus transcriptomics
• Dissertation : Development of poxvirus-based vectored vaccine platform for use in swine

 

 

 

 

 

Graduate Research Assistant

South Dakota State University

Jan 2015 – Jun 2018 SD

Responsibilities :

• Picronavirus pathogenesis
• Senecavirus A evolution
• Vectored vaccine development
• Next generation sequencing
• Thesis : Molecular epidemiology and pathogenesis of Senevavirus A

 

 

 

 

 

Research Intern

Regional Veterinary Lab

Aug 2013 – Feb 2014 Pokhara, Nepal

Responsibilities :

• Veterinary diagnostics
• Antibiotic resistance in animals
• Avian influenza outbreak surveillance
• Thesis :Prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) in the dairy farms of Pokhara, Nepal

Skills

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Mammalian cell culture	Confocal microscopy	Western blotting
DNA Cloning	Protein expression	Immunoprecipitation
Reverse genetics	Monoclonal antibody production	In-situ hybridization
Plaque assay	Animal models of viral diseases	Viral metagenomics
Virus isolation	Vaccine trials in animal models	RNA-Seq
Vaccine development	BSL-2, BSL-3 viral pathogen	R programming
PCR, RT-PCR, RACE PCR	ELISA, IFA,VN,HA/HI	Linux

Projects

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All Virus pathogenesis Vaccine development Viral genomics Diagnostics

Orf virus as a vaccine vector

Poxvirus-based vectored vaccine development

RNA-Seq studies

Using RNA-Seq to understand virus gene expression

Next generation sequencing

Using NGS tools for various projects.

Pathogenesis of Picornavirus

Pathogensis of swine picornaviruses.

Viral Diagnostic Assay Developement

Developing monoclonal and polyclonal antibodies

Publications

To get a complete list of publications click here.

Poxvirus vectors

The utility of poxviruses as expression vectors was first described in the early 1980s. Since then, poxviruses have been widely used as …

**Lok Raj Joshi**, Diego G Diel

PDF Source Document

Genetic diversity and evolution of the emerging picornavirus Senecavirus A

Abstract Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide.

**Lok R Joshi**, Kristin A. Mohr, Danielle Gava2, Gerald Kutish, Alaire S. Buysse, Fabio A. Vannucci, Pablo E. Piñeyro, Beate M. Crossley, John J. Schiltz, Melinda Jenkins-Moore, Leo Koster, Rachel Tell, Rejane Schaefer, Douglas Marthaler, Diego G Diel

PDF Project Source Document

A Novel Live Attenuated Vaccine Candidate Protects Against Heterologous Senecavirus A Challenge

Abstract Senecavirus A (SVA) is an emerging picornavirus causing vesicular disease (VD) clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no vaccines currently available for SVA. Here we developed a recombinant SVA strain (rSVAm SacII) using reverse genetics and assessed its immunogenicity and protective efficacy in pigs.

Bishwas Sharma, Maureen H.V. Fernandes, **Lok R.Joshi**, Steve Lawson, Diego G.Diel

PDF Source Document

Deletion of the thymidine kinase gene attenuates Caprine alphaherpesvirus 1 in goats

Highlights •Deletion of the tk gene from the CpHV-1 genome attenuates the virus in goats. •Traditional infection/transfection resulted in efficient CpHV-1 recombination. •Both parental and tk-deleted viruses established latency in neurons of the trigeminal ganglia.

Jéssica Caroline Gomes Noll, **Lok RajJoshi**, Gabriela Mansanodo Nascimento, Maureen Hoch Vieira Fernandes, Bishwas Sharma, Eduardo Furtado Flores, Diego Gustavo Diel

Source Document

Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States

Abstract Here we describe the identification and genetic characterization of a porcine hepe-astrovirus, or bastrovirus, obtained from feces from pigs in the United States. The genome of the new bastrovirus is 5,955 nt long and contains two open reading frames (ORFs).

Fernando V. Bauermann, Ben Hause, Alaine R. Buysse, **Lok R. Joshi**, Diego G. Diel

Project Source Document

See all publications

Posts

R package for pairwise gene comparision

This little package will help you to do perform a pairwise alignment of open reading frames of the two genomes. Generally, we use full genomes for pairwise comparison. Sometimes, it is interesting to look at the gene level to figure out where within the genome the variation is largest.

Jul 12, 2020 3 min read Virus genome

Fasta to Table to Fasta Conversion in R

Conversion of Fasta format to Tabular format and vice versa

Aug 11, 2019 1 min read

Working with the genome of non-model organism in R Bioconductor

GenomicFeatures and BSgenome are two widely used packages for genomic data analysis in R Bioconductor. They have variety of functions to work with genome features. Unfortunately, there are only a limited species you can work with BSgenome.

Aug 5, 2019 5 min read R

Package to design primers for RACE PCR

Description You can use this package to design 3' and 5’primers for Race PCR. The design is based on the method described by Clonetech Race PCR manual. The resulting primers will have vector sequence automatically added to them.

Jul 12, 2019 1 min read

Download CSV into R Studio from Github

You can use “readr” package to download CSV file and R data file from the github. Steps Go to the github repository link where you have the CSV file

Jan 9, 2019 1 min read R

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Awards

Awards

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Travel award to attend Systems Biology of Infectious Disease Workshop, Scripps Inst. (2020)

Diversity Schlorship from R Studio to attend rstusio::conf(2019)

SDSU Sigma XI Best PhD Paper Award (2018)

Bioimaging Contest-SDSU, Third Position (2018)

Best Oral Presentation Award, CRWAD Meeting (2016)

SDSU Sigma XI Grad Student Paper Award, Second Position (2016)

SDSU Sigma XI Grad Student Proposal Award,Second Position (2015)

First Award on National Level Story Writing Competition (2009),Radio Audio and USAID, Nepal

Merit Scholarship (2008-2013), Tribhuvan University Nepal

First Award on Essay Competition (2006), NIST College, Nepal

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Citations

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Gallery

Popular Topics

-R shiny -PCR -Cloning antibody BSgenome Coronavirus ELISA Fasta Genes Genome GenomicFeatures genomics Growth Curve Illumina Immune response Neutralizing antibodies NGS Orf virus pathogenesis PCR poxvirus R R shiny RNA-Seq Senecavirus vaccine Virus

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