Lok Raj Joshi
Biography
Interests
- AAV gene therapy
- Viral immunology
- Vaccine development
- Viral pathogenesis
- Diagnostic assay development
- Viral Bioinformatics
Education
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PhD in Biological and Biomedical Sciences (Molecular Virology), 2021
Cornell University
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Masters in Biological Science, 2017
South Dakota State University
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Bachelors in Veterinary Science (DVM), 2013
Tribhuvan University
Experience
Graduate Research Assistant
Cornell University
Responsibilites :
- Orf virus based vectored vaccine development
- Monoclonal antibody development
- SARS-CoV-2 pathogenesis
- Poxvirus transcriptomics
- Dissertation : Development of poxvirus-based vectored vaccine platform for use in swine
Graduate Research Assistant
South Dakota State University
Responsibilities :
- Picronavirus pathogenesis
- Senecavirus A evolution
- Vectored vaccine development
- Next generation sequencing
- Thesis : Molecular epidemiology and pathogenesis of Senevavirus A
Research Intern
Regional Veterinary Lab
Responsibilities :
- Veterinary diagnostics
- Antibiotic resistance in animals
- Avian influenza outbreak surveillance
- Thesis :Prevalence of Methicillin-resistant Staphylococcus aureus (MRSA) in the dairy farms of Pokhara, Nepal
Skills
| Mammalian cell culture | Confocal microscopy | Western blotting |
| DNA Cloning | Protein expression | Immunoprecipitation |
| Reverse genetics | Monoclonal antibody production | In-situ hybridization |
| Plaque assay | Animal models of viral diseases | Viral metagenomics |
| Virus isolation | Vaccine trials in animal models | RNA-Seq |
| Vaccine development | BSL-2, BSL-3 viral pathogen | R programming |
| PCR, RT-PCR, RACE PCR | ELISA, IFA,VN,HA/HI | Linux |
Projects
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Orf virus as a vaccine vector
Poxvirus-based vectored vaccine development
RNA-Seq studies
Using RNA-Seq to understand virus gene expression
Next generation sequencing
Using NGS tools for various projects.
Pathogenesis of Picornavirus
Pathogensis of swine picornaviruses.
Viral Diagnostic Assay Developement
Developing monoclonal and polyclonal antibodies
Publications
To get a complete list of publications click here.
Poxvirus vectors
The utility of poxviruses as expression vectors was first described in the early 1980s. Since then, poxviruses have been widely used as …
Genetic diversity and evolution of the emerging picornavirus Senecavirus A
Abstract
Senecavirus A (SVA) is an emerging picornavirus that causes vesicular disease (VD) in swine. The virus has been circulating in swine in the United Stated (USA) since at least 1988, however, since 2014 a marked increase in the number of SVA outbreaks has been observed in swine worldwide.
A Novel Live Attenuated Vaccine Candidate Protects Against Heterologous Senecavirus A Challenge
Abstract
Senecavirus A (SVA) is an emerging picornavirus causing vesicular disease (VD) clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no vaccines currently available for SVA. Here we developed a recombinant SVA strain (rSVAm SacII) using reverse genetics and assessed its immunogenicity and protective efficacy in pigs.
Deletion of the thymidine kinase gene attenuates Caprine alphaherpesvirus 1 in goats
Highlights
•Deletion of the tk gene from the CpHV-1 genome attenuates the virus in goats.
•Traditional infection/transfection resulted in efficient CpHV-1 recombination.
•Both parental and tk-deleted viruses established latency in neurons of the trigeminal ganglia.
Identification and genetic characterization of a porcine hepe-astrovirus (bastrovirus) in the United States
Abstract
Here we describe the identification and genetic characterization of a porcine hepe-astrovirus, or bastrovirus, obtained from feces from pigs in the United States. The genome of the new bastrovirus is 5,955 nt long and contains two open reading frames (ORFs).
Posts
R package for pairwise gene comparision
This little package will help you to do perform a pairwise alignment of open reading frames of the two genomes. Generally, we use full genomes for pairwise comparison. Sometimes, it is interesting to look at the gene level to figure out where within the genome the variation is largest.
Working with the genome of non-model organism in R Bioconductor
GenomicFeatures and BSgenome are two widely used packages for genomic data analysis in R Bioconductor. They have variety of functions to work with genome features. Unfortunately, there are only a limited species you can work with BSgenome.
Package to design primers for RACE PCR
Description You can use this package to design 3' and 5’primers for Race PCR. The design is based on the method described by Clonetech Race PCR manual. The resulting primers will have vector sequence automatically added to them.